I dont have got much expertise with ELISAs, but I do realize that the calibration figure are usually sigmoidal and linear regression will not provide a great ur2 value.
Elisa Data Analysis Excel Software Program ToI do not have the software program to create a 4-parameter logistic contour suit Can anyone tell me if I can obtain a general ELISA quantitation template from someplace Also, what contour fit do you make use of I possess comtemplated using 2-order polynomial but after that I am not sure how to apply the equation in Excel tó quantitate my unknówns because I am not certain how to make use of some of the terms, for instance: con 2E-09x3 - 4E-06X2 0.0024x 0.01 (what can be the worth of Y) Any help will be higly appreciated -ElsaElsa-.Get rid of the factors with a high absorbance that perform not drop on a direct series and story it.Four and fivé parameter logistic curves are usually to my information only useful when making use of aggressive ELISAs.
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